Amino Acid Analysis
Amino Acid (composition) Analysis
of a protein or
is in general performed to determine the protein concentration
, or protein content.
In combination with UV measurement, however, it can also be used to get the
molar extinction coefficient
. This analysis is used throughout all stages of drug discovery and development, and/or to demonstrate comparability and consistency between batches for release testing during the manufacturing phase. Amino acid analysis is a key part of
the ICH Q6B guidelines
for characterisation and
confirmation of biopharmaceuticals in support of new marketing applications.
All kind of samples is supported, viz.: dry, in solution, and even in SDS-PAGE gel (see technical info
). Also we offer D,L separation and quantification. Since a highly sensitive RP-HPLC and fluorescence based method is used, a minimum quantity of only about 2 µg of protein material will already give good results. Determination of free amino acids
in cell culture media
, or for certain Food / Feed
applications is also offered. See also our technical / background